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Volume  2 (2024)
Volume  1 (2023)
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VOLUME 1 , ISSUE 2 ( July-December, 2023 ) > List of Articles

Original Article

Impact of Oocytes with Mild and Heavy Debris in Perivitelline Space on Blastocyst Quality

V Dinesh Ram, N Chandan, Mahalakshmi, Jayashree Srinivasan, J Damodharan

Keywords : Human menopausal gonadotropin, Perivitelline space, Zona pellucida

Citation Information : Ram VD, Chandan N, Mahalakshmi, Srinivasan J, Damodharan J. Impact of Oocytes with Mild and Heavy Debris in Perivitelline Space on Blastocyst Quality. J Obstet Gynaecol 2023; 1 (2):42-48.

DOI: 10.5005/jogyp-11012-0014

License: CC BY-NC 4.0

Published Online: 24-11-2023

Copyright Statement:  Copyright © 2023; The Author(s).


Abstract

Aim: The aim of this study is to compare the blastocyst quality of oocytes with mild and heavy debris in perivitelline space (DPVS). Objective: To investigate the rate of blastocyst development of oocytes with mild DPVS and to investigate the rate of blastocyst development of oocytes with heavy DPVS, then to compare the rate of blastocyst development of oocytes with mild and heavy DPVS and to identify ideal blastocyst for transfer. Study population: Patients who have undergone controlled ovarian stimulation for the intracytoplasmic sperm injection (ICSI) cycle from March 2023 to May 2023 ARC International Fertility and Research Centre. Study groups: Two groups were considered—oocytes with mild debris or granularity in perivitelline space (PVS) and oocytes with heavy debris or granularity in PVS. Results: This study from March 2023 to May 2023 was done at ARC International Fertility and Research Centre. Patients undergoing the Antagonist protocol for the ICSI cycle were considered. Around 371 M2 oocytes were analyzed, in that 203 had mild debris or granularity in PVS and 168 had heavy debris or granularity in PVS. In oocytes with mild debris or granularity in PVS, their fertilization rate, cleavage rate, and frozen blastocyst rate are 88, 81, and 47%, respectively. In oocytes with heavy debris or granularity in PVS their fertilization rate, cleavage rate, and frozen blastocyst rate are 76, 59, and 15%, respectively. Oocytes with heavy debris or granularity in PVS have compromised blastocyst quality compared with mild debris or granularity in PVS. Conclusion: In this study, we conclude that debris or granularity in PVS can be considered an indicator of oocyte competence. This can help in identifying a cohort of oocytes with a lower chance of forming viable embryos. This helps in reducing the culture of supernumerary embryos. We suggest that when the patients enroll for donor oocytes, oocytes without heavy debris or granularity in PVS should be used because heavy granularity in PVS has compromised blastocyst conversion. Further studies are needed with a large sample size to get a conclusion.

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